Bethesda Assay

Test Name:

Bethesda Assay

Description:

Test: Bethesda Assay
MRP: 9155
Parameters:
Method: Clot Based
Sample Type: 4 ml. (3 ml. Minimum) PPP (Platelet Poor Plasma) from 2 Sodium Citrate tubes prepared within 1 hr of collection. Aliquot PPP in 2 transport vials and FREEZE.
Instruction: Preparation of PPP : 12 -14 Hrs. (Overnight) fasting is preferred. Centrifuge the blood sample at 3000rpm for 10 minutes within 1 hr. of collection to separate the plasma. Transfer the Plasma obtained to a clean plastic tube using plastic tipped pipette avoiding buffy coat & centrifuge it again at 3700 rpm & finally transfer the PPP into fresh, clean & labelled plastic tubes which contains <10,000 cells/cu. mm. DULY FILLED COAGULATION REQUISITION TO BE ATTACHED
TAT: 3rd Working Day by 7 m
Department: Haematology

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